Fresh and Frozen B Cells from Peripheral Blood
CD19+ B Cells are isolated from peripheral blood mononuclear cells by positive CD19 selection or by depletion of other cell types. For additional B Cell subsets, such as Naïve B Cells, CD138+ B Cells, or Memory B Cells, please inquire here. All cells are obtained using Institutional Review Board (IRB) approved consent forms and protocols.
|4570-41||CD19+ B Cells [5 x 10^6, negative selection]|
|4570-42||CD19+ B Cells [5 x 10^6, positive selection]|
|4585-11||CD19+ B Cells [5 x 10^6, negative selection]|
|4585-12||CD19+ B Cells [5 x 10^6, positive selection]|
Fresh isolated cell orders must be submitted a minimum of one week prior to the requested delivery date. Additional lead time may be required for custom cell types or specific donor requests. If there is a preference for cells isolated from whole blood, cord blood, or apheresis, please list in order notes.
Certificate of Analysis & References
B cells are isolated from PBMC by positive immunomagnetic isolation using CD19 microbeads or negative selection through depletion of other cell types. CD19+ cell products come with a standard certificate of analysis including basic donor information, cell count, and flow data. Flow data comes with a label check instead of anti-CD19 antibody for positively selected cells due to antigen saturation during positive selection. Final cell concentration, cryopreservation medium, and custom flow panels may all be requested here.
Cells are generally isolated from PBMC from leukopaks. If you would like them isolated from whole blood or LRS chamber, you request starting material at time of order.
Donors are infectious disease tested annually and are free of Hepatitis B, Hepatitis C, HIV-1, and HIV-2. For more information, please contact us.
All cells are obtained using Institutional Review Board (IRB) approved consent forms and protocols and standard phlebotomy practices.
Shipping and Delivery
FEDEX PRIORITY OVERNIGHT
(only outside of Greater Seattle area. Charged to your individual FedEx account):
Samples will be shipped overnight to your shipping address, arriving before 10:30am to most US addresses. You will receive tracking notifications from FedEx via email when the package has been picked up and when it’s been delivered.
(within Greater Seattle area):
Delivery to your location is by same-day FedEx courier in the morning, guaranteed to arrive within 2–3 hours of the sample ready time, depending on your location. You will receive tracking notifications from FedEx via email when the package has been picked up and when it’s been delivered.
Change orders and cancellation notices on all orders must be submitted to Bloodworks ([email protected]) and receive acknowledgement in writing and change orders must be mutually agreed upon. Cancellations of products in the following categories will result in the following fees:
|Fresh Isolated and Custom Frozen Cells||Less than 2 weeks||75% of list or quoted price|
|Less than 3 business days||100% of list or quoted price|
|Rescheduling Fee||50% of list or quoted price|
All change fee and cancellation policies for special projects or indicated in Scope of Work documents supersede the above listed fees. Rescheduling subject to donor availability. Postponements or new requests with a rush requirement of less than 3 business days may be subject to an additional $100 rush fee.