Human Peripheral Blood Mononuclear Cells (PBMCs)
Peripheral blood mononuclear cells (PBMCs) are nucleated cells (monocytes, lymphocytes, dendritic cells, and progenitor cells) collected from peripheral blood by density gradient centrifugation.
All PBMCs come with a Certificate of Analysis, limited donor information, flow profile, and microscopic observations. PBMCs from TRIMA filters are subject to limited donor information and cannot be used for whole genome sequencing.
All cells are obtained using Institutional Review Board (IRB) approved consent forms and protocols.
|4520-01||Leukopak PBMCs [platet washed, 2.5 x 10^9]|
|4520-02||Leukopak PBMCs [platet washed, 5 x 10^9]|
|4520-03||Leukopak PBMCs [platet washed, 8-12 x 10^9]|
|4525-01||Leukopak PBMCs [ficoll, 1.5 x 10^9]|
|4525-02||Leukopak PBMCs [ficoll, 3 x 10^9]|
|4525-03||Leukopak PBMCs [ficoll, 5-8 x 10^9]|
|4570-01||Whole Blood PBMCs [ficoll, 150 x 10^6]|
|4570-02||Whole Blood PBMCs [ficoll, 250 x 10^6]|
|4570-03||Leukopak TRIMA filter PBMCs [500 x 10^6]|
|4580-01||PBMCs [25 x 10^6]|
|4580-02||PBMCs [50 x 10^6]|
|4580-03||PBMCs [100 x 10^6]|
|4580-04||PBMCs [200 x 10^6]|
Fresh PBMC orders must be submitted a minimum of one week prior to the requested delivery date.
Additional lead time may be required for custom concentrations or specific donor requests.
If there is a preference for cells from cord blood, please list in order notes.
Certificate of Analysis & Literary References
PBMC are from apheresis product by density gradient centrifugation. Fresh, frozen, and fresh frozen are available. Final cell concentration, cryopreservation medium, and custom flow panels may all be requested here.
If you are interested in PBMC from whole blood or LRS chamber, you request starting material at time of order.
Donors are infectious disease tested annually and are free of Hepatitis B, Hepatitis C, HIV-1, and HIV-2. For more information, please contact us.
All cells are obtained using Institutional Review Board (IRB) approved consent forms and protocols and standard phlebotomy practices.
Certificate of Analysis
PBMC Certificate of Analysis
James, C.A., Xu, Y., Aguilar, M.S. et al. CD4 and CD8 co-receptors modulate functional avidity of CD1b-restricted T cells. Nat Commun 13, 78 (2022). https://doi.org/10.1038/s41467-021-27764-w
Bolouri, H., Young, M., Beilke, J. et al. Integrative network modeling reveals mechanisms underlying T cell exhaustion. Sci Rep 10, 1915 (2020). https://doi.org/10.1038/s41598-020-58600-8
Simonich C, Shipley MM, Doepker L, Gobillot T, Garrett M, Cale EM, Hennessy B, Itell H, Chohan V, Doria-Rose N, Nduati R, Overbaugh J. A diverse collection of B cells responded to HIV infection in infant BG505. Cell Rep Med. 2021 Jun 15;2(6):100314. doi: 10.1016/j.xcrm.2021.100314.
Kim CC, Baccarella AM, Bayat A, Pepper M, Fontana MF. FCRL5+ Memory B Cells Exhibit Robust Recall Responses. Cell Rep. 2019 Apr 30;27(5):1446-1460.e4. doi: 10.1016/j.celrep.2019.04.019.
Shipping and Delivery
FEDEX PRIORITY OVERNIGHT
(only outside of Greater Seattle area. Charged to your individual FedEx account):
Samples will be shipped overnight to your shipping address, arriving before 10:30am to most US addresses. You will receive tracking notifications from FedEx via email when the package has been picked up and when it’s been delivered.
(within Greater Seattle area):
Delivery to your location is by same-day FedEx courier in the morning, guaranteed to arrive within 2–3 hours of the sample ready time, depending on your location. You will receive tracking notifications from FedEx via email when the package has been picked up and when it’s been delivered.
Change orders and cancellation notices on all orders must be submitted to Bloodworks ([email protected]) and receive acknowledgement in writing and change orders must be mutually agreed upon. Cancellations of products in the following categories will result in the following fees:
|Fresh Isolated and Custom Frozen Cells||Less than 2 weeks||75% of list or quoted price|
|Less than 3 business days||100% of list or quoted price|
|Rescheduling Fee||50% of list or quoted price|
|Fresh PBMCs||Less than 3 business days||25% of list or quoted price|
All change fee and cancellation policies for special projects or indicated in Scope of Work documents supersede the above listed fees. Rescheduling subject to donor availability. Postponements or new requests with a rush requirement of less than 3 business days may be subject to an additional $100 rush fee.